Graft of the NT-3 persistent delivery gelatin sponge scaffold promotes axon regeneration, attenuates inflammation, and induces cell migration in rat and canine with spinal cord injury

摘要

Persistent neurotrophic factor delivery is crucial to create a microenvironment for cell survival and nerve regeneration in spinal cord injury (SCI). This study aimed to develop a NT-3/fibroin coated gelatin sponge scaffold (NF-GS) as a novel controlled artificial release therapy for SCI. In vitro, bone marrow -derived mesenchymal stem cells (MSCs) were planted into the NF -GS and release test showed that NF -GS was capable to generate a sustainable NT-3 release up to 28 days. MSCs in NF -GS had high cell activity with excellent cell distribution and phenotype. Then, the NF -GS was transplanted into the injury site of spinal cord of rat and canine in vivo, which exhibited strong biocompatibility during post -transplantation period. Four weeks following transplantation, the concentration of NT-3 was much higher than that in control groups. Cavity areas in the injury/graft site were significantly reduced due to tissue regeneration and axonal extensions associated with myelin sheath through the glial scar into the NF -GS. Additionally, the NF -GS decreased the inflammation by reducing the CD68 positive cells and TNF-alpha. A striking feature was the occurrence of some cells and myelin -like structure that appeared to traverse the NF -GS. The present results demonstrate that the NF -GS has the property to control the release of NT-3 from the NT-3/fibroin complex thus facilitating regeneration of injured spinal cord. (C) 2015 Elsevier Ltd. All rights reserved.