11#beta#-Hydroxysteroid Dehydrogenase Type 1 from Human Liver: Dimerization and Enzyme Cooperativity Support Its Postulated Role as Glucocorticoid Reductase

摘要

11#beta#-Hydroxysteropid dehydrogenase type 1 (11#beta#-HSD 1) is a microsomal enzyme that catalyzes the reversible interconversion of receptor-active 11-hydroxy glucocorticoids (cortisol) to their receptorinactive 11-oxo metabolites (cortisone). However, the physiological role of 11#beta#-HSD 1 as prereceptor control device in regulating access of glucocorticoid hormones to the glucocorticoid receptor remains obscure in light of its low substrate affinities, which is in contrast to low glucocorticoid plasma levels and low K_d values of the receptors to cortisol. To solve this enigma, we performed detailed kinetic analyses with a homogeneously purified 11#beta#-HSD 1 from human liver. The membrane-bound enzyme was successfully obtained in an active state by a purification procedure that took advantage of gentle solubilization method as well as providing a favorable detergent surrounding during the various chromatographic steps. The identity of purified 11#beta#-HSD 1 was proven by determination of enzymatic activity, N-terminal amino acid sequencing, and immunoblot analysis. By gel-permeation chromatography we could demonstrate that 11#beta#-HSD 1 is active as a dimeric enzyme. The cDNA for the enzyme was cloned from a human liver cDNA library and shown to be homologous to that previously characterized in human testis. Interestingly, 11#beta#-HSD 1 exhibits Michaelis-Menten kinetics with cortisol and corticosterone (11#beta#-dehydrogenation activity) but cooperative kinetics with cortisone and dehydrocorti-costerone (11-oxoreducing activity). Accordingly, this enzyme dynamically adapts to low (nanomolar) as well as to high (micromolar) substrate concentrations, thereby providing the fine-tuning required as a consequence of great variations in circadian plasma glucocorticoid levels.