Effect of chemical mutagens and gamma irradiation on the in vitro culture of inbred maize lines.

摘要

Frequencies of callus production and regeneration were studied in the lines BC2923, Pioneer 346, Pioneer 502 and Oh43 under various cultural conditions, including application of 3 chemical mutagens and gamma rays. Optimum concentration of the chemicals, radiation dose and mode of treatment for the induction of callus and regeneration of plantlets were determined. The rate of callus formation depended on the mutagen used. Regenerants obtained after the use of mutagens were weaker than somaclonal regenerants. Methods for combining somaclonal and mutational variation were devised.