Role of nitric oxide in the SOS-signal formation in Escherichia coli

摘要

Having one electron with unpaired spin, nitric oxide (NO) shows high reactivity and activates or inhibits free radical chain reactions. NO toxic and genotoxic effects appear to be the result of intracellular formation of peroxinitrite that can inducesome cellular damages, including DNA strand breaks. DNA base oxidation, destruction of the key enzymes, etc. Taking into account the character of DNA damages being formed under NO activity, we proposed a formation of the SOS signal and induction the SOSDNA repair response in E. coli cells treated with NO physiological donors - DNIC and GSNO. The ability of NO donor compounds to induce the SOS DNA response in E. coli PQ37 with sfiA::lacZ operon fusion is reported here at the first time. So, the SOS DNArepair response induction is one of the function of nitric oxide.