New recombinant strain of Escherichia coli BL21 ECC13SLP, new expression vectors and mode of transformation of Escherichia coli BL21

摘要

modified bacterial genome revealed that contains heterologiczne genes dehydratazy glicerolowej subsidiary from vitamin b12, reaktywatora dehydratazy glicerolowej 1,3 - pdo and oksydoreduktazy as vectors ekspresyjnych.genes encoding subunits dehydratazy glicerolowej originate from the bacterium citrobacter freundii, genes for subunits reaktywatora dehydratazy glicerolowej originate from citrobacter freundii and oksydoreduktazy 1,3 - pdo gene from the bacteria klebsiella pneumoniae.vectors expressive pcf1 and pkp2 contain individual genes heterologiczne originating in bacterium citrobacter freundii and klebsiella pneumoniae respectively and are oflankowane regulatorowymi sequences. in the transformation of e. coli for transformation using new vectors expressive pcf1 and pkp2.