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首页> 外文期刊>Bioprocess Engineering: Bioreactors Upstream and Downstream Processes Measurement and Control >An alternative approach to the detection of lignin: a note on the application of ELISA using polyclonal antibodies
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An alternative approach to the detection of lignin: a note on the application of ELISA using polyclonal antibodies

机译:木质素检测的另一种方法:使用多克隆抗体的ELISA应用说明

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摘要

A specific detection method for lignin has been developed using polyclonal antibodies raised against BSA coupled lignin. This method was found to be highly sensitive and linear in enzyme linked immunosorbant assay (ELISA) within the coating lignin concentration range of 0.01 mu g/ml to 1 mu g/ml. The interference in the detection due to the presence of lignin breakdown products and other coexisting biopolymers could be eliminated at an antiserum dilution of 1:25600. [References: 10]
机译:已经使用针对BSA偶联木质素的多克隆抗体开发了木质素的特异性检测方法。发现该方法在包被的木质素浓度范围为0.01μg/ ml至1μg/ ml的酶联免疫吸附测定(ELISA)中是高度灵敏的和线性的。以1:25600的抗血清稀释可以消除由于木质素分解产物和其他共存生物聚合物的存在而对检测产生的干扰。 [参考:10]

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