IMPROVEMENT OF THE METHOD FOR MUSTELIDAE SPECIES IDENTIFICATION BY DNA ISOLATED FROM FECES

摘要

An improved method for the Mustelidae species identification using mtDNA isolated from the samples of feces is proposed. New primers for the amplification and sequencing of a fragment of mtDNA were used; a methodology for the DNA extraction from mustelid feces was developed; a successful identification using pre-known samples and samples from the wildlife was shown. The species was identified in more than 80% of the samples, which is the highest rate in such studies. The use of new more successful primers will permit to simplify investigation of Eurasian Mustelidae.