X-Ray Diffraction and Nuclear Magnetic Resonance in the Structural Genomics Study of Helicobacter Pylori

摘要

The three-dimensional structure of protein is an essential topic for the biochemical function research in recent year. X-ray diffraction and nuclear magnetic resonance (NMR) are two important methods in this field. In this article, we will introduce the principles and circumstance of these two methods, and how we apply these two methods in our study: Helicobacter pylori structural genomics research. Proteins carry out their biological functions only when they fold correctly. A unique protein folding respects to have a specific biological function. So, we can investigate the three-dimensional structure of protein to study the possible biological function of protein. We determine the active site of protein and the key residues involved in the biological function. The resolution of these methods is in atomic resolution.