Effect of mitochondria on the redox reaction between oxymyoglobin and ferricytochrome c

摘要

The effect of mitochondria on the redox reaction between oxymyoglobin (oxy-Mb) and ferricytochrome c was studied. The parameters of this reaction in the absence of mitochondria have been investigated earlier. It is shown that the course of oxidation of oxymyoglobin by cytochrome c in the presence of mitochondria differs from that without mitochondria: no reduced cytochrome c is observed; in addition, the order of this redox reaction and its dependence on pH and ionic strength change. The factors influencing the state of mitochondrial membrane and uncouples enhance markedly the reaction rate. The conclusion was drawn that imtochondria directly participate in the oxymyoglobin-cytochrome c redox reaction. The possibility of this reaction in vivo under extreme conditions and during pathological processes is discussed.