A new method for introducing enzymes into cellulosic matrixes which can be formed into membranes,films,or beads has been developed using a cellulose-in-ionic-liquid dissolution and regeneration process.Initial results on the formation of thin cellulose films incorporating dispersed laccase indicate that active enzyme-encapsulated films can be prepared using this methodology and that precoating the enzyme with a second,hydrophobic ionic liquid prior to dispersion in the cellulose/ionic liquid solution can provide an increase in enzyme activity relative to that of untreated films,presumably by providing a stabilizing microenvironment for the enzyme.
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