Synthesis and Characterization of Silklike Materials Containing the Calcium-Binding Sequence from Calbindin D9k or the Shell Nacreous Matrix Protein MSI60

摘要

Artificial bone-and dentinlike biomaterials for tissue regeneration are of considerable potential interest in clinical medicine.Bones and dentin are mainly composed of a combination of inorganic hydroxyapatite(HA)nanocrystals and the protein type I collagen.Hydroxyapatite formation is accelerated in vitro by certain specific extracellular matrix macromolecules acting as nucleators,while in vivo certain acidic noncollagenous proteins are thought to play an important role in hydroxyapatite deposition.Accordingly,many investigations are focused on artificial composite materials constructed from hydroxyapatite and proteins or synthetic polymers.