Effect of Overexpression of Proline Transporter ProP on High Saline Adaptation in Escherichia Coli.

摘要

The effect of the amount of proline transporter ProP expression on the mechanism of quick adaptation of Escherichia coli cells under high salinity was analyzed. The ProP gene derived from E. coli expression plasmid was introduced into E. coli cells, and a ProP high expression strain was developed. Under 1.2 M NaCl culture conditions, the growth of normal E. coli cell was inhibited, whereas ProP high expression cells showed remarkable growth. The uptake of proline by E. coli as a compatible solute accorded well with those of cell growth. These data suggested that the amount of proline transporter ProP expression played an important role in quick adaptation of E. coli cells under 1.2 M NaCl culture conditions.