末梢血幹細胞採取時期決定における多項目自動血球分析装置SE-9000の有用性

摘要

We evaluated the usefulness of an automated hematology analyzer, SE-9000, to determine the optimal day for harvesting peripheral blood stem cells. Both immature information (IMI) positive cells (IMI+ cells) and hematopoietic progenitor cell (HPC) positive cells (HPC+ cells), which were obtained from the IMI scatter gram of the analyzer, correlated well with CD34 + cells in peripheral blood. Although IMI+ cells sometimes showed false positive data due to toxic granulation of neutrophils and other factors, HPC+ cells apparently did not produce these errors. Thus, the scatter gram should be checked carefully, when IMI+ cells are used. IMI or HPC data obtained immediately before peripheral blood stem cell harvest showed significant correlation with the number of harvested CD34 cells, and could be used to determine the optimal time for harvest.