In vitro reproduction of the catagen like apoptosis on the hair follicle keratinocytes

摘要

We have previously reported that in the catagen phase apoptotic cells increased, compared with those in the anagen phase. Although many groups have tried to reveal the mechanism of induction of apoptosis, this is still unclear because there is no method to reproduce the catagen like apoptosis (CLA) on the hair follicle keratinocytes (HFK) in vitro. In this report, to induce the CLA on the HFK, we have examined whether apoptosis occurred on the HFK by disruption of attachment to the culture dish. Moreover, the effect of the caspase inhibitors on the CLA induced by such method was examined. The HFK were cultured on agarose coated dish for complete suspension culture and fragmentated DNA was analyzed. Fragmentated DNA was also analyzed after addition of the caspase inhibitors to this suspension culture. As the results, fragmentated DNA from the HFK cultured on the agarose coated dish was increased, compared with that on the type I collagen coated dish. Moreover, fragmentated DNA was completely disappeared by addition of the caspasel/caspasel like protease inhibitor. All these results suggest that, in part, the CLA may be reproduced on the HFK by disruption of attachment to the culture dish and some caspases may regulate apoptosis in the catagen phase.