Phosphorus Composition and Phosphatase Activities in Soils Affected by Long-Term Application of Pig Manure and Inorganic Fertilizers

摘要

The long-term (25 years) effect of using chemical fertilizers and animal manure on soil phosphorus (P) composition and phosphatase activities was investigated in this study. Results showed that pig manure applications significantly increased soil total P, Olsen P, and phosphatase activities, whereas chemical fertilizers had no significant effects on soil chemical properties and phosphatase activities. Manure applications doubled or tripled the orthophosphate concentrations as compared to chemical fertilizers. Analysis of solution P-31 nuclear magnetic resonance (NMR) spectroscopy showed that P composition in sodium hydroxide (NaOH)-ethylenediamenetetraacetic acid (EDTA) extracts was dominated by orthophosphate (59-84%), followed by phosphomonoesters (15-40%). More organic P (P-o), especially myo-inositol hexakisphosphate, was observed in soil treated with manure as compared with soil treated with chemical fertilizer