The Liquefaction Effects of Crude-Actinidin Treatments with Heating on Adult Cattle Aponeuroses Collagen under acidic conditions [Japanese]

摘要

About 1% of cattle aponeuroses was extracted after 60 min of heating in hot water at 85degreesC. The collagen subunit chains containing beta- and alpha-chains were not detected in the insoluble fractions of cattle aponeuroses after 60 min heating at 80degreesC by SDS-PAGE analysis. The insoluble fractions were treated with plant thiol proteases such as crude-actinidin, papain, bromelain and ficin at 70degreesC for 60 min under acid conditions, and then the effects of extraction from the treated-cattle aponeuroses were analyzed by measuring the yield,of total and extracted-collagen. The percentage of extraction from the cattle aponeuroses treated with crude-actinidin, papain, bromelain or ficin was at a level of 34.7, 11.7, 6.6, and 2.8% yield, respectively (p<0.01). Furthermore, collagen subunit chains were detected in the heated-aponeuroses (85degreesC, 60 min) treated with crude-actinidin at 37degreesC for 3 h in 0.5 M acetic acid, but not detected in the un-heated aponeuroses extracted with 0.5 M acetic acid at 37degreesC for 3 h.