Bioremediation of waste cooking oil using a novel lipase produced by Penicillium chrysogenum SNP5 grown in solid medium containing waste grease

摘要

The aim of present work was to bioremediate the waste cooking oil using a novel lipase produced in solid medium containing waste grease and wheat bran by Penicillium chrysogenum. Enzyme extracted with phosphate buffer was purified 10.6 and 26.28-fold after 90% ammonium sulfate precipitation and ion-exchange chromatography, respectively. The partial characterization of enzyme revealed its K _m and V _(max) value for p-nitrophenolpamitate as 0.4mM and 47.61U/ml, respectively. The relative molecular mass of lipase was 40kDa by SDS-PAGE and confirmed by zymogram. Purified lipase was most stable at 40°C and at 8.0 pH. Lipase activity was enhanced by metal ions such as Mg ~(2+), Fe ~(2+), Ca ~(2+) and non-ionic surfactant TritonX-100, while suppressed in the presence of SDS. Crude lipase was applied on cooking oil waste and the acid value was 26.92mg/g. This showed that the enzyme could be employed for the bioremediation of used cooking oil.