RAPID SCREENING AND DETERMINATION OF NERVE AGENT METABOLITES IN HUMAN URINE BY LC一MS/MS

摘要

Qualitative screening procedures have been developed for the rapid detection and identification of the metabolites of nerve agents in the urine samples and extracts using liquid chromatography—tandem mass spectrometry (LC—MS/MS). The combination ofnegative electrospray ionization (ESI) using a CI8 column and water—methanol mobile phase modified with ammonium formate provides a rapid screening procedure for nerve agent degradation products with limit of detection of1ng/mL in the precursor-ion analysis. Also, determination of the alkyl methylphosphonic acids was carried out by the SRM scan mode with the limit of detection of 0.1ng/mL亍hese procedures will be applicable to the trace analysis of metabolites of nerve agents in human urine matrices inthe Organisation for the Prohibition of Chemical eapons (OPCW) proficiency test.