Cloning and expression of the streptavidin gene from streptomyces avidinii in escherichia coli and secretion of streptavidin by E.coli cells

摘要

The streptavidin gene from Streptomyces avidinii was cloned, an expression plasmid constructed, and a highly effective strain producer of streptavidin created. It was shown that the leader peptide of streptavidin ensures the effective secretion of this protein into the periplasmic space of Eschericha coli cells. The degradation site of the leader peptide was detected. Upon treatment with the total fraction of proteases secreted by S.avidinii into the culture medium, "core" streptavidin was obtained, which retained the biotin-binding function.