Callus formation and regeneration of adventitious embryos from celery microspores by anther and isolated microspore cultures.

摘要

Anthers and microspores of Apium graveolens were cultured in vitro to obtain haploid plants. For anther culture, immature anthers of cultivars Cornell 619 and Cornell 19 at 3 stages of development were cultured on 8 media containing half-strength MS(1/2 MS) and B5 media supplemented with combinations of 2,4-D, BA, NAA and zeatin plus sucrose and Gelrite. Calluses developed from Cornell 19 microspores at the tetrad stage on 1/2 MS medium supplemented with 2,4-D and B5 medium supplemented with 2,4-D. In Cornell 619, calluses developed from tetrad and early uninucleate microspores on B5 medium supplemented with 2,4-D. Adventitious embryos regenerated from calluses transferred to MS medium with or without NAA and BA; plantlets grew after 40 to 60 days. For isolated microspore culture, microspores at different developmental stages were cultured in liquid B5, NLN and 1/2 MS media supplemented with 2,4-D and BA, with or without glutamine and with serine at a density of 2.5 X 103 microspores/ml medium.In Cornell 619, many microspores at the early uninucleate stage developed into colonies in B5, NLN and 1/2 MS media; the colonies in B5 and 1/2 MS media developed into heart-shaped embryos. Microspores plated in modified 1/2 MS medium supplemented with 2,4-D and BA developed colonies and calluses.