Selective Anti-Hepatoma Treated with Titanium Oxide Nanoparticles in vitro

摘要

Effects of titanium oxide (TiO_2) nanoparticles on Bel-7402 human hepatoma cells and L-02 human hepatocytes at different times were observed. Using cell culture, cell growth curves of Bel-7402 cells and L-02 cells treated with TiO_2 nanoparticles were examined by MTT assay, and the cellular ultrastructure was observed by an analytical transmission electron microscope (ATEM). It is found that OD value of Bel-7402 cell treated with TiO_2 nanoparticles for 48-144h is obviously lower than that of control group (p < 0.01). However the growth curve of L-02 cells is almost not affected by TiO_2 nanoparticles. ATEM and energy dispersive X-ray (EDX) analyses show that there are obvious vacuoles increased heterolysosome, and particles with high electron density which are confirmed to be TiO_2 nanoparticles in Bel-7402 cytoplasm. More interestingly, it is alse found that TiO_2 nanoparticle obviously inhibits the proliferation of hepatoma cells by altering lysosome activity and destroying cytoplasm structure. The inhibition on proliferation of hepatocytes by TiO_2 nanoparticles is much slighter. The results demonstrate that TiO_2 nanoparticle has different killing effects on cancer cell and normal cell.