BACTERIAL COMMUNITY COMPOSITIONS OF EJECTED INTESTINE OF JUVENILE SEA CUCUMBER AND THE EFFECT OF SEA SEDIMENT BACILLUS ON IN VIVO ANTAGONISM AGAINST PATHOGENIC VIBRIO SPLENDIDUS

摘要

Along with the rapid expansion of sea cucumber culture of China, the occurrence of bacterial diseases has been the bottleneck for sustainable development. Pathogens Vibrio splendidus, V. harveyi, and V. cyclitrophicus have been isolated from skin ulceration disease and viscera ejection syndrome of sea cucumber A. japonicas (Deng et al., 2009). On the basis of 16S rRNA gene pyrose-quencing, we analyzed bacterial community compositions of ejected intestine of juvenile sea cucumber and coastal sea sediment. We formulated a mixture of Bacillus subtilis, B. amyloliquefaciens, and B. megaterium from coastal sea sediment as probiotic candidates. Their probiotic effects on in vivo antagonism against pathogenic V. splendidus was evaluated.