Effect of Sample Storage on Blood Crossmatching in Horses

摘要

Background Blood samples banked for up to 1 similar to month are typically used to perform pretransfusion testing in humans and small animals, but this has not been validated using blood from horses. Hypothesis Compatibility of equine blood samples is repeatable using fresh samples, and reproducible using donor blood samples stored for up to 4 similar to weeks. Animals Six healthy adult horses. Methods Randomized, blinded experimental study. Immunologic compatibility of the blood of all horses was assessed using a major and minor saline agglutination and hemolysin crossmatch using blood samples refrigerated for 04 similar to weeks and fresh blood from the same horses. Crossmatch results were scored and then compared to identify changes of compatibility in each of the 4 tests. In addition, repeatability of the crossmatch technique itself was assessed by performing 6 iterations of this procedure in immediate succession with fresh blood from 3 horses. Results No significant difference in crossmatch results was found using fresh blood (P similar to=similar to.391.00). Reproducibility was poor using blood stored for 14 similar to weeks, especially in tests using stored erythrocytes (major antigen crossmatches), with significant differences from baseline at all weeks (P similar to