FUMONISIN CONCENTRATION AND CERAMIDE SYNTHASE INHIBITORY ACTIVITY OF CORN, MASA, AND TORTILLA CHIPS

摘要

Nixtamalization removes fumonisins from corn and reduces their amounts in masa and tortilla products Fumonisin concentrations and potential toxicity could be underestimated, however, if unknown but biologically active fumonisins are present. Therefore, the relative amounts of tumonisins in extracts of fumonisin-contaminated corn and its masa and tortilla chip nixtamalization products were determined with an in vitro ceramide synthase inhibition bioassay using increased sphinganine (Sa) and sphinganine to sphingosine ratio (Sa/So) as endpoints. African green monkey kidney cells (Vero cells ATCC CCL-81) were grown in 1-ml wells and exposed to 4 mul of the concentrated extracts for 48 h. The corn extract inhibited ceramide synthase as Sa (mean = 112 pmol/well) and Sa/So (mean = 2 24) were high compared to vehicle controls (5a = 9 pmol/well; Sa/So = 0 10) Inhibitory activity (mean Sa = 14-24 pmol/well; mean Sal So = 0.17-0,28) of the masa and tortilla chip extracts were reduced >80% compared to the corn extract. Results were corroborated in a second experiment in which Sa and Sa/So of the wells treated with masa or tortilla chip extracts were reduced >89% compared to those treated with the corn extract. Masa and tortilla chip FB_1 concentrations (4-7 ppm) were reduced about 80-90% compared to the corn (30 ppm) when the materials were analyzed by high-performance liquid chromatography (HPLC) Therefore, nixtamalization reduced both the measured amount of FB_1 and the ceramide synthase inhibitory activity of masa and tortilla chips extracts. The results further suggest that the masa and tortilla chip extracts did not contain significant amounts of unknown fumonisins having ceramide synthase inhibitory activity.