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Simulation of confocal microscopy through scattering media with and without time gating

机译:在有时间选通和没有时间选通的情况下通过散射介质对共聚焦显微镜进行模拟

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摘要

An efficient and fast simulation technique is presented to calculate characteristic features of confocal imaging through scattering media. The simulation can predict the time-resolved confocal response to pulsed illumination that allows optimizing of imaging contrast when time-gating techniques are applied. Modest computational effort is sufficient to obtain contrast predictions for arbitrary numerical aperture, focus depth, pinhole size, and scattering density, while the simulation accuracy is independent of scattering density and pinhole size. In the case of isotropic scattering, our results indicate that reflection-mode confocal imaging through scattering media is limited to mud approximate to 3.5 optical thicknesses for continuous-wave illumination. If time-gating is applied, imaging through scattering densities of mud approximate to 8 is theoretically possible. (C) 2001 Optical Society of America. [References: 19]
机译:提出了一种高效,快速的模拟技术,通过散射介质计算共焦成像的特征。该仿真可以预测对脉冲照明的时间分辨共聚焦响应,从而在应用时间门控技术时可以优化成像对比度。适度的计算工作足以为任意数值孔径,聚焦深度,针孔尺寸和散射密度获得对比度预测,而仿真精度与散射密度和针孔尺寸无关。在各向同性散射的情况下,我们的结果表明,通过散射介质的反射模式共聚焦成像仅限于连续波照明约3.5光学厚度的泥浆。如果应用时间选通,则理论上可以通过接近8的泥浆散射密度进行成像。 (C)2001年美国眼镜学会。 [参考:19]

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