Positional Recognition and Attitude Control for 3-D Biomicromanipulation in Microscopy

摘要

The biomicromanipulation so important to biology and bioengineering remains difficult because object used are very small, kept in liquids, or observed by optical microscopy. We are developing a micromanipulation system for anatomical operation of microobjects such as embryos, cells, and microbes. Microscope images are 2-D, so it is difficult to manipulate targets in 3-D space. To improve manipulation work, we proposed a 3-D biomicromanipulation system combined with virtual reality (VR) space. Here we propose the 3-D modeling method of the object to present 3-D visual information to the operator and improve the operation environment. In this system, we still have difficulty changing the orientation of the microscopic object by manipulating a mechanical manipulator. The bioaligner we propose is a microdevice for the positional control of an object. We developed a 2-D bioaligner by microfabrication. Here we show its fabrication process and basic rotating experiment with chlorella cells.