Separation and purification of six compounds from the flowers of Cerasus yedoensis (Matsum.) by high-speed countercurrent chromatography in stepwise elution mode

摘要

Six compounds from the flower of Cerasus yedoensis (Matsum.) were successfully isolated by high-speed countercurrent chromatography (HSCCC) and preparative high performance liquid chromatography using stepwise elution with a pair of two-phase solvent systems composed of ethyl acetate-n-butanol-formic acid-water at volume ratio of 4:1.5:0.15:5 and ethyl acetate-ethanol-formic acid-water at volume ratio of 4:1:0.15:5 for the first time. This separation process produced (a) 141mg of 1-O-caffeoyl--D-glucopyranoside, (b) 28mg of p-coumaric acid glucoside, (c) 13mg of chlorogenic acid, (d) 21mg of quercetin-3-O--D-glucopyranoside, (e) 19mg of kaempferol 3-O--D-glucopyranoside, and (f) 25mg of caffeic acid from 400mg of crude sample with the purities of 96.51, 98.82, 94.96, 99.01, 82.51, and 82.45%, respectively. MS, H-1 NMR, and C-13 NMR analyses were used for the chemical structure identification.