Determination of two textile dyes in wastewater by solid phase extraction and liquid chromatography/electrospray ionization tandem mass spectrometry analysis

摘要

This study describes a procedure for the determination of two textile dyes (Nylosan Red N-2RBL-azo and Optilan Blue MF-GL-anthraquinone) from wastewater by solid phase extraction and liquid chromatography/electrospray ionization tandem mass spectrometry (SPE-LC/ESI-MS/MS) analysis. Four types of Strata SPE cartridges (C18-U, C18-E, WAX/NH2, and SAX) were tested; the best results being obtained on Strata WAX/NH2. The detection of dyes was carried out by mass spectrometry with an API 3200 QTRAP ESI-MS/MS system using a TurboV ionization source in ESI negative ion mode. The method of multiple reaction monitoring transitions was followed to quantify the precursor molecular ions and the related product ions. A mobile phase of methanol:water (70/30, v/v) at 0.65 mL min-1 flow rate was used for elution. The procedure was validated by: linearity, limits of detection and quantification, accuracy, and precision. The obtained detection/quantification limits were: 0.285/0.864 ng mL-1 for Nylosan Red N-2RBL and 0.433/1.310 ng mL-1 for Optilan Blue MF-GL. The presented procedure has been applied for monitoring the studied textile dyes in influent and effluent wastewater from a treatment plant from an area in Romania that has a textile factory.