Preparative separation of phenolic constituents in the fruits of Luffa cylindrica (L.) roem using slow rotary countercurrent chromatography

摘要

In the present study we performed preparative separations of the phenolic compounds in two pre-purified sponge gourd extracts Fr30 and Fr50 from macro-porous resin AB-8, using slow rotary countercurrent chromatography (SRCCC) with two solvent systems, respectively. The separation of 3 g of Fr30 using solvent system CHCl3-MeOH-2-propanol -H2O (5:6:1:4, v/v) yielded five compounds: p-coumaric acid (1, 65 mg), 1-O-feruloyl-beta-D-glucose (2, 189 mg), 1-O-p-coumaroyl-beta-D-glucose (3, 96 mg), 1-O-caffeoyl-beta-D-glucose (4, 130 mg), and 1-O-(4-hydroxybenzoyl)-glucose (5, 90 mg), and the separation of 3 g of Fr50 using solvent system CHCl3-MeOH-H2O (13:7:8, v/v) afforded three compounds: diosmetin-7-O-beta-D-glucuronide methyl ester (6, 125 mg), apigenin-7-O-beta-D-glucuronide methyl ester (7, 164 mg), and luteolin-7-O-beta-D-glucuronide methyl ester (8, 98 mg). The excellent results demonstrate that SRCCC is an efficient method to prepare large amounts of natural products.