Preparative separation of saponins from the Luffa cylindeica (L.) Roem. by slow rotary countercurrent chromatography

摘要

Slow rotary countercurrent chromatography (SRCCC) was used for the separation of saponins from towel gourd crude extract (Luffa cyclindria Roem.) with a biphasic solvent system composed of chloroform-methanol-water (13:7:8, v/v). In each separation of SRCCC, 3 g crude saponins sample yielded 357 mg Lucyoside Q (1) and 213 mg Lucyoside H (2). The structures of the two compounds were confirmed by means of ESI-MS, H-1- and C-13-NMR.