Simple UHPLC-DAD Method for the Direct Determination of Donepezil in Cerebrospinal Fluid

摘要

Donepezil is an acetylcholinesterase inhibitor administered to patients who suffer from Alzheimer's Disease (AD). We have currently developed and validated a UHPLC-DAD method for the determination of donepezil in cerebrospinal fluid. The retention time of the drug was 2.4min and the total time of analysis was 6min. Validation was performed in terms of linearity, selectivity, accuracy, precision, and stability. Following validation, the method was applied to clinical cerebrospinal fluid samples. The currently developed method is a reliable tool for monitoring CSF donepezil levels in patients under treatment with this drug.