Fast and simple method for the determination of chemotherapeutic agents in biological fluids and infusion equipment based on them

摘要

Method for determining the concentration of a chemotherapeutic agent and its possibly present metabolites in a biological fluid sample of an individual at a point in time between 0 and 60 minutes after the start of administration of the chemotherapeutic agent or one of its salts to the individual, the chemotherapeutic agent being selected from the group consisting of Doxorubicin, epirubicin, idarubicin, daunorubicin, etoposide, 5-fluorouracil, fludarabine and chlorambucil, comprising i) the separation of proteins contained in the liquid sample by precipitating the proteins with a precipitation reagent and centrifugation or filtration of the liquid sample, the precipitation reagent being selected from the group consisting of trichloroacetic acid, methanol, ethanol, acetonitrile, zinc sulfate and mixtures of two or more of these substances, andii) measuring the concentration of a chemotherapeutic agent and its possibly present metabolites in a biological one Liquid sample by means of a fluorescence signal emanating from the liquid sample after protein separation upon irradiation with excitation light, characterized in that the method does not include any chromatographic or electrophoretic process steps and no liquid-liquid extraction.