Temperature Controlled Restrictor for Packed Capillary Column Supercritical Fluid Chromatography

摘要

Fatty acid methyl esters were separated by a packed capillary supercritical fluid chromatographic (SFC) column and detected by a UV detector at the wavelength of 206 nm. The temperature controlled restrictor was designed for UV detection. The temperature controlled restrictor is a 20 cm length of deactivated fused silica of 7 jxm i.d., which is held right after UV detector of the packed capillary SFC. The temperature of the restrictor will control the flow rate of the supercritical carbon dioxide mobile phase through the packed capillary column in SFC. Thus, as the pressure in the column is increased during a pressure program, the temperature of 7 mum fused silica tube can be varied to maintain a constant flow rate.