Capillary-LC-fjtESI-MS/MS and Nano-LC-Nano ESI-MS/MS Analysis Using a Single Binary Pump Capillary LC System: Applications in Proteomics

摘要

We describe the use of a single pump capillary-LC system (flow rate: l-100muJL/min) to perform both capillary-LC-muESI-MS/MS and nano-LC-nano ESI-MS/MS (flow rate: 50-1000nL/min) analysis and its applications in proteomics. A highly constant nanoflow (180nL/min) delivery has been achieved by using a capillary binary-pump with electronic flow control and a flow splitter with compensation for the viscosity changes during gradient elution.