Robotic Sample Preparation and HPLC Determination of the Major Component of Ivermectin in Human Plasma

摘要

A robotic sample preparation method combined with high performance liquid chromatography (HPLC)-fluorescence detection for the determination of the major component of ivermectin in human plasma is presented. A Cyberlab C-300 workstation equipped with customized software and hardware was utilized to perform all the semi-automated liquid-solid phase extraction (LSPE) steps including acetonitrile (ACN) and water addition, sample loading, and hexane elution on Chem Elut~(TM) cartridges. Under the automated pre-colmn derivatization conditions, both ivermectin and internal standard of the extracted samples were chemically converted to highly fluorescent derivatives and then monitored by a fluorescence detector. The assay was validated in the concentration range of 0.5-40 ng/mL, using 1 mL of human plasma. The application of this assay was demonstrated by analysis of plasma samples from human subjects receiving oral doses of ivermectin.