Isolation and Purification of Psoralen and Bergapten from Ficus carica L. Leaves by High-Speed Countercurrent Chromatography

摘要

High-speed countercurrent chromatography (HSCCC) was successfully applied for the first time for the isolation and purification of psoralen and bergapten from the plant Ficus carica L leaves. The crude extract was obtained by extraction with light petroleum (b.p.: 60degC-90degC) from the dried leaves of Ficus carica L. M-Hexane-ethyl acetate-methanol-water (1:1:1:1, v/v) was used as the two-phase solvent system. Each peak fraction was analyzed by high performance liquid chromatography (HPLC). The method yielded 4.4 mg of psoralen at 99.1% purity and 2.1 mg of bergapten at 98.2% purity from 400 mg of the crude extract in a single run. The structures of the two compounds were identified by 'H-NMR, 13C-NMR, and MS.