Nine high purity of flavonoid glycosides and caffeoylquinic acid derivatives were isolated and purified by combination of HSCCC and prep-HPLC from leaves of Lonicera japonica Thunb. The n-butanol extract was firstly isolated by HSCCC using methyl tent-butyl ether-n-butanol-acetonitrile-water (0.5% acetic acid) (2:2:1:5,v/v), yielding five fractions F1,F2 (rhoifolin), F3 (luteoloside), F4 and FS (collected from the column after the separation). The sub-fractions F1, F4 and FS were separated by pre-HPLC successfully. Finally, chlorogenic acid (1), lonicerin (2), rutin (3), rhoifolin (4), luteoloside (5), 3, 4-O-dicaffeoylquinic acid (6), hyperoside (7), 3, S-O-dicaffeoylquinic acid (8) and 4, S-O-dicaffeoylquinic acid (9) were yielded,with the purities over 94%, respectively, as determined by HPLC.
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