Departments of Chemistry and Animal Sciences,University of Illinois at Urbana-Champaign,Urbana,Illinois 61801

摘要

For proteomic monitoring of processes such as allergy or inflammation an efficient pre-fractionation strategy is required.We isolated plasma membranes from human peripheral blood mononuclear(PBM)cells by aqueous two-phase partitioning.After 1DE combined with LC-MS/MS,several cell surface marker proteins and in total 60 different plasma membrane proteins(out of 84 identified proteins,i.e.,72%)were detected.Plasma membranes obtained were from only one human donor,the procedure is therefore applicable for individual patient screening.