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首页> 外文期刊>Journal of Pure & Applied Microbiology >Feather Keratin Degradation by Stenotrophomonas sp AB20 Screened from Poultry Waste
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Feather Keratin Degradation by Stenotrophomonas sp AB20 Screened from Poultry Waste

机译:从家禽粪便中筛选嗜麦芽单胞菌sp AB20对羽毛角蛋白的降解作用

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Microbial keratinase is biotechnologically important enzyme which has ability to hydrolyze highly rigid, strongly cross-linked structural polypeptides as well as recalcitrant and insoluble fibrous keratin proteins. In the present investigation, 25 feather waste dumped soil samples were screened for keratinolytic bacteria. Samples were enriched in mineral salt medium containing 1% feather followed by screening for protease activity using casein agar, milk agar and keratinase acitivity with feather meal agar. Amongst 82 bacterial isolates, 39 showed maximum proteolytic and 17 showed keratinolytic activity. Moreover isolate Stenotrophomonas sp. AB 20 showed highest potential in both the proteolytic and keratinolytic activities. In addition to this, isolate AB 20 showed more than 87.3% feather degradation within 6 days using submerged degradation process and crude enzyme activity was 52.82 U/ml. Identification of potential keratinolytic isolate trough 16S rRNA analysis revealed that it belongs to Stenotrophomonas sp. (Gene bank accession number KF201645). The isolate due to its potential keratinolytic activity may serve as a candidate for feather waste management and number of different biotechnological and industrial applications.
机译:微生物角蛋白酶是生物技术上重要的酶,具有水解高度刚性,强交联的结构多肽以及顽固和不溶性纤维状角蛋白的能力。在本研究中,对25个羽毛废料倾倒的土壤样品进行了角蛋白分解细菌的筛选。将样品富集在含1%羽毛的矿物盐培养基中,然后使用酪蛋白琼脂,牛奶琼脂和带有羽毛粉琼脂的角蛋白酶活性筛选蛋白酶活性。在82种细菌分离物中,有39种具有最大的蛋白水解活性,有17种具有角蛋白分解活性。此外,分离到嗜麦芽单胞菌。 AB 20在蛋白水解和角蛋白水解中均显示出最高的潜力。除此之外,使用淹没降解过程,分离株AB 20在6天内显示出超过87.3%的羽毛降解,粗酶活性为52.82 U / ml。潜在的角蛋白分解分离物槽16S rRNA分析的鉴定表明,它属于Stenotrophomonas sp。 (基因库登录号KF201645)。该分离物由于其潜在的角蛋白分解活性而可以用作羽毛废物管理以及许多不同的生物技术和工业应用的候选物。

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