New Safety and Rapid Method for Extraction of Genomic DNA from Bacteria and Yeast Strains Suitable for PCR Amplifications

摘要

Molecular biology studies based on PCR amplification require the extraction of good quality genomic DNA. In this study, freeze- sudden thawing as a safety, new, quick and low-cost genomic DNA extraction technique from gram-negative and gram-positive bacteria, and Saccharomyces and non- Saccharomyces yeasts for PCR applications has been developed. This technique does not require any risky chemicals, enzymes and/or extra purification steps. DNA extracted by this method is suitable for PCR applications using random and specific primers such as RAPD-PCR for fingerprinting and DNA sequencing of the 16S rDNA or the D1/D2 domain of the 26S rDNA for molecular identification of bacterial and yeast isolates respectively