Experimental Research on Pigment Epithelium Derived Factor joint Lens Injury Promote Optic Nerve Regeneration in Rats

摘要

This study investigated whether pigment epithelium derived factor (PEDF) combined with lens injury protection coordination of rat retinal ganglion cells (RGCs) survival and promote RGCs axonal regeneration purposes, using Fluoro-Gold microinjection into adult SD rats and 2 W SD was born in brain of young rats, retrograde labeled RGCs and preparation of optic nerve injury models and crystalline lens injury model, observation number method, draw a result, intravitreal injection of PEDF lens injury canincrease RGCs survival number, both of which can be coordinated so that this role increased. Lens injury stimulates axon regeneration, combined with PEDF can enhance the stimulation of regeneration. Lens injury and PEDF can improve GAP-43 mRNA expression, the combination of the two makes this action to strengthen.