TRYPSIN INHIBITOR ACTIVITY IN SOYBEAN SEEDS AND SOYBEAN PRODUCTS

摘要

Studies on the antitrypsinic activity in raw soybean seeds (the varieties Zarya. Beesson and Hodson). soybean meals (from Bulgaria and produced in Argentina) and soybean flakes have been conducted. The trypsin inhibitor activity (TlU/mg) has been determined according to the AACC method (2003) with slight modificationThe investigation was carried out to comparative measurements of the trypsin inhibitor activity in raw soybean seeds and soybean products at two wavelengths which is related to the possibility of increasing the sensitivity and decreasing the spectrophotometer error of the method.The absorbtional spectrum of the enzyme reaction product (p-nitroaniline) against the reference solution possesses maximum of absorption at 388nm. while the DL-BAPNA shows continuum in the range from 380nm to 420nm, under the same conditions. The absorbance of p-nitroaniline, measured at 388nm is by 38.6% higher than the absorbance measured at 4'iOnm. The results shows that a spectrophotometer error of 1nm at the lower wavelength a relative error of 0.1% whereas the similar instrumental error at 41 On in gives 3.1 %The comparative measurements on the trypsin inhibitor activity (TlU/mg) in soybean seeds and soybean products at 410nm and 388nm show higher values by 31-33% at the lower wavelengthThe detail measurements on trypsin inhibitor activity. which are the antinutritional factors in soybean; are of great importance for their processing related to the supply of high quality protein components of the animal and human food.