Use of Two-Dimensional TLC to Identify Phenolic Acids in the Foliage and Fruit of Peucedanum tauricum Bleb

摘要

Two-dimensional planar chromatography (2D TLC) on cellulose layers, solid phase extraction (SPE), and reversed-phase high-performance liquid chromatography (HPLC) have been used for purification, separation, and identification of the phenolic acids in the foliage and fruits of Peucedanum tauricum Bieb. (Umbelliferae = Apiaceae). Methanolic extracts of the foliage and fruit were purified and the free phenolic acids fractions were isolated by means of the classical method of Ibrahim and Towers. Hydrolysis of the esters and glycosides was performed by the method of Schmidtlein and Herrmann. Isolated fractions containing phenolic acids were investigated by comparison with standards, by means of analytical 2D TLC on cellulose layers, by, use of derivatization reagents (gaseous NH_3, 3% FeCl3 and diazotized sulfanilic acid in 20% sodium carbonate solution), and by RP HPLC with isocratic elution (mobile phase methanol-water, 20:80 (v/v) containing 1% (v/v) acetic acid) and UV spectroscopy (by means of the diode-array detector of the liquid chromatograph).By use of this procedure the cinnamic acid derivatives caffeic acid,p- coumaric acid, and ferulic acid, the benzoic acid derivatives vanillic acid,p-hydroxybenzoic acid, protocatechuic acid, gentisic acid, and syringic acid, and the depside chlorogenic acid were detected and identified in the foliage of Peucedanum tauricum Bieb. Caffeic, p- coumaric, vanillic, p-hydroxybenzoic, protocatechuic, and syringic acids were also detected and identified in the fruit of the plant. This is the first time these phenolic acids have been isolated, detected, and identified in the foliage and fruit of P. tauricum Bieb.