Development of a Loop-Mediated Isothermal Amplification Assay to Detect Fusarium oxysporum

摘要

We report the development of a loop-mediated isothermal amplification (LAMP) assay targeting the CYP51C element for visual detection of F.oxysporum which caused Fusarium wilt in soybean. The CYP51C-LAMP assay efficiently amplified the target gene in 60min at 62 degrees C. And specificity was evaluated against F.oxysporum, Fusarium spp. and other fungal species. The detection limit of the CYP51C-specific LAMP assay for F.oxysporum was four conidia per gram soil. The assay also detected F.oxysporum from inoculated soybean tissues and residues. These results suggest that this CYP51C-LAMP assay can be used to detect residues on plants in the field.