Natural Occurrence of Wild tomato mosaic virus in Wild Eggplant in China

摘要

In winter 2013, typical symptoms of green crinkling and mosaic were observed on wild eggplant leaves in field in Hainan, China. The causal pathogen was identified to be a Wild tomato mosaic virus (WTMV) based upon reverse transcription-touchdown PCR with degenerate primers. A specific product of 1700bp containing the partial sequence of the NIb (950bp) and CP (750bp) coding regions of WTMV was amplified, and the predicted polypeptide consisted of 572 amino acids (Mr 65.52 kDa). This isolate was subsequently named WTMV-Hainan (GenBank accession ) isolate, and it shared sequence identities of 91% and 95% with WTMV-Laichau isolate at the nucleotide and the amino acid levels, respectively. Phylogenetic analysis of the CP gene sequences provided further evidence that all WTMV isolates formed a high-confidence subclade and WTMV-Hainan most closely related to WTMV-KAN, WTMV-Laichau and WTMV-GD1. To our knowledge, this is the first report of the natural infection of WTMV on wild eggplant in China.