PHYLOGENETIC CHARACIERIZAnON OF HANTAVIRUSES FROM WILD RODENTS IN SOUTHEAST COASTAL REGION OF CHINA, 1981—2007

摘要

Objective To investigate the genomic variability of Hantavirus (HV) isolates from different hosts and different locations in southeast coastal region of China in 1981—2007. Methods The entire M and S segments of the HV genome were amplified using reverse transcription (RT) polymerase chain reaction (PCR) and were analyzed by Phylogenetic trees using DNAstar and MEGA 4. 0. Result ()f the 12 HV strains, five were classified as HTNV and the others as SEOV. In HTNV isolates from the same region shared high degree of sequence identity and clustered on the same branch of Phylogenetic trees respectively while isolates from different districts were clustered on the adjacent branches. Of the 7 strains of SEOV, two isolates from Jiande county in Zhejiang province were clustered on a separated branch, which has wider sequences variability than the others. Conclusion The HV sequence variation may owe more to the regional than temporal or host disparity, particularly for HTNV.