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Investigations of conformational transitions in proteins and RNA using 2DCOS Raman and 2DCOS Raman optical activity spectroscopies

机译:使用2DCOS拉曼和2DCOS拉曼旋光光谱研究蛋白质和RNA的构象转变

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Coupling the structural sensitivity of vibrational spectroscopics, such as Raman scattering and Raman optical activity (ROA), with the analytical insight provided by 2D generalized correlation analysis provides a new and exciting set of tools for structural biology. These new tools of 2DCos Raman and 2DCos ROA have the potential to provide new information on the mechanisms of conformational transitions of proteins and ribonucleic acids (RNA), such as those for folding and misfolding. However, the complexity of biomolecular transitions and the practicalities of spectroscopic data collection can make the analysis of 2DCos Raman and 2DCos ROA contour plots difficult. In this paper we present it summary of our methodology for obtaining reliable 2DCos contour maps and for their interpretation for folding and misfolding transitions in polypeptides, proteins and RNA. We demonstrate that our protocols for data pre-treatment greatly improve the quality of 2DCos contour maps, revealing the large amount of structural information that they contain, and then show that a moving window analysis is required to adequately follow biomolecular conformational transitions in detail. (C) 2007 Elsevier B.V. All rights reserved.
机译:将振动光谱仪的结构灵敏度(例如拉曼散射和拉曼光学活性(ROA))与2D广义相关分析提供的分析见解结合起来,为结构生物学提供了一组令人兴奋的新工具。 2DCos拉曼和2DCos ROA的这些新工具具有提供有关蛋白质和核糖核酸(RNA)构象转变机制的新信息的潜力,例如折叠和错误折叠的机制。然而,生物分子跃迁的复杂性和光谱数据收集的实用性使2DCos拉曼和2DCos ROA等高线图的分析变得困难。在本文中,我们对获得可靠的2DCos等高线图及其对多肽,蛋白质和RNA中折叠和错误折叠过渡的解释进行了总结。我们证明了我们用于数据预处理的协议极大地提高了2DCos等高线图的质量,揭示了它们包含的大量结构信息,然后表明需要进行移动窗口分析才能充分详细跟踪生物分子构象转变。 (C)2007 Elsevier B.V.保留所有权利。

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