Genetic Diversity Analysis of PVY Resistant Potato Varieties (Clones) by RAPD Markers

摘要

DNA fingerprints of 37 PVY resistant potato varieties (clones) were generated using PCR-based RAPD analysis. All 37 varieties were differentiated by banding patterns obtained from 17 primers that generated 164 polymorphisms. Similarity matrices were calculated with the simple matching coefficient. The genetic distance value among them was between 0.27 and 0.50, with average value being 0.35. The average link clustering algorithm was used to construct a dendrogram with Statistica. They were classifiedinto three groups according to clustering results, and the results presented some geographic relationship. Dendrogram showed close genetic relationships between a number of varieties (clones) of similar pedigree. This study has shown that RAPD marker isa useful tool for potato variety identification, differentiation, and estimation of genetic relationships.