首页> 外文会议>International Agro-Biotechnology Conference; 2005; Heilongjiang(CN) >Genetic Diversity Analysis of PVY-resistant Potato Accessions by RAPD Makers
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Genetic Diversity Analysis of PVY-resistant Potato Accessions by RAPD Makers

机译:RAPD基因对耐PVY马铃薯种质遗传多样性的分析

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DNA fingerprints of 37 PVY-resistant potato accessions were generated using PCR-based RAPD analysis. All 37 culti-vars were differentiated by banding patterns obtained from 17 primers that generated 164 polymorphisms. Similarity matrices were calculated with the simple matching coefficient. The genetic distance value among them was between 0. 27 and 0. 50, average value was 0. 35. and the average link clustering algorithm was used to construct a dendrogram with STATISTICA. They were classified into three groups according to clustering results, and the results presented some geographic relationship. Dendrogram showed close genetic relationships between a number of cultivars of similar pedigree. This study has shown that RAPD markers is a useful tool for potato cultivar identification, differentiation, and estimating genetic relationships.
机译:使用基于PCR的RAPD分析生成了37个耐PVY马铃薯种质的DNA指纹图谱。通过从产生164个多态性的17个引物获得的条带模式来区分所有37个品种。用简单匹配系数计算相似度矩阵。它们之间的遗传距离值在0. 27和0. 50之间,平均值是0. 35.,并且使用平均链接聚类算法用STATISTICA构建树状图。根据聚类结果将它们分为三类,结果呈现出一定的地理关系。树状图显示了许多相似谱系的品种之间密切的遗传关系。这项研究表明,RAPD标记是马铃薯品种鉴定,分化和估计遗传关系的有用工具。

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