基于RAPD标记的云南23个八角优良无性系的聚类和遗传多样性分析

摘要

利用RAPD分子标记技术对云南省的23个八角优良无性系进行了聚类和遗传多样性分析.从筛选出的22条引物中,共扩增出197个位点,其中多态性位点183个,平均多态性比率为92.89％.采用成对算术平均数的非加权成组配对法(UPGMA)对Nei's的一致度进行的聚类分析结果表明,在23个八角无性系中,广南7号与广南9号的遗传距离最近,广南9号与富宁3号的遗传距离最远.在遗传距离为0.142 5处将23个八角无性系分为4个类群.试验数据表明,其八角无性系间的遗传距离与地理距离的相关性不大.遗传多样性分析结果表明,云南23个八角优良无性系间存在较高的遗传多态性和丰富的遗传基础.%Using 23 selected superior clones of Illicium verum from Yunnan province as the materials, random amplified polymorphic DNA (RAPD) markers were applied to analyze the genetic diversity. Totally 197 bands were amplified by 22 selected primers, among which 183 bands were polymorphic with the polymorphic loci percentage of 92. 89 % . Dendrogram of 23 clones was constructed by the way of unweighted pair-group method (UPMGA) based on Nei' s genetic identity, 23 clones were therefore clustered into four main groups at genetic distance of 0. 142 5. The results showed that genetic distance between Guangnan No. 7 and Guangnan No. 9 was the smallest, while which of Guangnan No. 9 and Funing No. 3 was the largest. The analysis results implied that the genetic distances of individuals had no obvious correlation to their geographic distances. The genetic diversity of the studied 23 clones of Illicium verum from Yunnan province was high and the genetic basis was rich.